產(chǎn)品英文名稱：Stemcell 19758 EasySep™ Mouse Epithelial Cell Enrichment Kit
產(chǎn)品中文名稱：Stemcell 19758 EasySep™ 小鼠上皮細(xì)胞富集試劑盒

Stemcell 19758 EasySep™ Mouse Epithelial Cell Enrichment Kit 產(chǎn)品簡介
Stemcell 19762 EasySep™ Mouse Neutrophil Enrichment Kit 旨在通過陰性選擇從新鮮解離的小鼠乳腺組織中分離上皮細(xì)胞。不需要的細(xì)胞有針對性的被非上皮細(xì)胞(CD4, CD5, CD11c, CD45R/B220, CD49b, CD117, TER119, F4/80)針對的生物素抗體瞄準(zhǔn)去除，。標(biāo)記的細(xì)胞然后被抗生物素和葡聚糖的四聚體抗體配合物識別。這些細(xì)胞被綁定到磁性粒子，使用沒有柱的EasySep™磁鐵 分離。期望得到的細(xì)胞倒入到一個新的管。

Stemcell 19758 EasySep™ 小鼠上皮細(xì)胞富集試劑盒產(chǎn)品組分
Stemcell 19758 EasySep™ 小鼠上皮細(xì)胞富集試劑盒有三個部分
• EasySep™ Mouse Epithelial Cell Enrichment Cocktail, 0.5 mL
• EasySep™ Biotin Selection Cocktail, 1.0 mL
• EasySep™ Magnetic Particles, 1.0 mL

Stemcell 19758 EasySep™ Mouse Epithelial Cell Enrichment Kit 推薦使用
Stemcell 19758 EasySep™ Mouse Epithelial Cell Enrichment Kit is designed for the isolation of mouse epithelial cells from cell suspensions by negative selection

Stemcell 19758 EasySep™ 小鼠上皮細(xì)胞富集試劑盒所需設(shè)備
• EasySep™ Magnet (Catalog #18000), or
• "The Big Easy" EasySep™ Magnet (Catalog #18001)

Stemcell 19758 EasySep™ Mouse Epithelial Cell Enrichment Kit 保存方法
EASYSEP™ MOUSE EPITHELIAL CELL ENRICHMENT COCKTAIL
Product stable at 2 - 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 25°C), and should be refrigerated upon receipt.
EASYSEP™ BIOTIN SELECTION COCKTAIL
Product stable at 2 - 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 25°C), and should be refrigerated upon receipt.
EASYSEP™ MAGNETIC NANOPARTICLES
Product stable at 2 - 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 25°C), and should be refrigerated upon receipt.

Stemcell 19758 EasySep™ Mouse Epithelial Cell Enrichment Kit 實驗資料
Start: 10.37 ± 0.50% of cells expressing one or more of the following markers: CD45, Ter119, CD31, and/or BP-1. Enriched: 1.12 ± 0.22% of cells expressing one or more of the following markers: CD45, Ter119, CD31, and/or BP-1.

The percentage of hematopoietic, endothelial, and fibroblast cells after separation is 1.12 ± 0.22%. Purity has been assessed by staining with goat anti-rat IgG-FITC, which recognizes the antibodies used to deplete cells expressing CD45, Ter119, CD31, and/or BP-1.

Typical EasySep™ Procedure for Mouse Cells


Stemcell 19758 EasySep™ 小鼠上皮細(xì)胞富集試劑盒相關(guān)產(chǎn)品訂購信息
Stemcell  07912  Collagenase/Hyaluronidase  10X Collagenase/Hyaluronidase in DMEM 
Stemcell  18000  EasySep™ Magnet  Immunomagnetic Column-Free Magnet 
Stemcell  18001  The Big Easy EasySep™ Magnet  Immunomagnetic Column-Free Magnet Stemcell  20000  RoboSep™  RoboSep™ - The Fully Automated Cell Separator 
Stemcell  05610  EpiCult™-B Mouse Medium Kit  Medium for Mouse Mammary Epithelial Cell Culture 
Stemcell  05640  ProstaCult™ Mouse Medium Kit  Medium for the Culture of Mouse Prostate Epithelial Cells     
For research use only. Not intended for human or animal diagnostic or therapeutic uses.