Cellartis DEF-CS?500 iPS無外源3D懸浮培養(yǎng)基

傳統(tǒng)的2D培養(yǎng)系統(tǒng)無法支持放大培養(yǎng)（Scale-up）所需細胞數(shù)量級別（> 109個細胞/處理）以維持細胞療法的增長需求。?3D懸浮培養(yǎng)是大規(guī)模生產(chǎn)多能細胞的可行經(jīng)濟有效的解決方案。盡管干細胞傳統(tǒng)上難以適應懸浮培養(yǎng)，但CellArtis研究人員已經(jīng)開發(fā)出一種培養(yǎng)體系，可以在成分確定、無飼養(yǎng)層和無異源物條件下實現(xiàn)穩(wěn)定高效的生長并維持多能性。Cellartis DEF-CS?500 iPS無外源3D懸浮培養(yǎng)基與生物反應器系統(tǒng)配套，為創(chuàng)建大量高質(zhì)量，未分化的起始材料提供了理想的方法。該培養(yǎng)基的配方基于2D培養(yǎng)基Cellartis DEF-CS 500 Xeno-Free Culture Medium，2D培養(yǎng)的細胞可以輕松過渡到3D以實現(xiàn)可靠的放大。

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英文名稱：Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium w/o Antibiotics

中文名稱：Cellartis DEF-CS?500無外源3D懸浮iPS培養(yǎng)基，不含抗生素

用途：iPS?細胞3D懸浮細胞治療所需10億級

保存：基礎培養(yǎng)基2-8°C，添加劑-20°C

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組份

基礎培養(yǎng)基+三種添加劑

Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium w/o Antibiotics (Cat. No. Y30047)

500 ml Cellartis DEF-CS 500 Xeno-Free Basal Medium w/o Antibiotics (Cat. No Y30046; not sold?separately)

Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Additives (Cat. No. Y30048)

o 500 μl DEF-CS Xeno-Free 3D Spheroid Additive 1 (1,000X)

o 200 μl DEF-CS Xeno-Free 3D Spheroid Additive 2 (4,000X)

o 400 μl DEF-CS Xeno-Free 3D Spheroid Additive 3 (500X)

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自行購買試劑

緩沖液和細胞分離試劑

PBS Dulbecco’s w/o Ca2+ & Mg2+ (D-PBS?–/–)

Accumax (Merck Millipore, Cat. No. SCR006)

可選抗生素和消化試劑

Gentamicin (Thermo Fisher, Cat. No. 15750)

Versene Solution (Thermo Fisher, Cat. No. 15040) (for transfer from monolayer culture)

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細胞培養(yǎng)參數(shù)

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Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium

Traditional 2D culture systems simply cannot support the scale-up required (>109 cells/treatment) to sustain the growing demand for cell-based therapies. 3D suspension culture is a viable, cost-effective solution for large-scale production of pluripotent cells. Although stem cells have traditionally been difficult to adapt to suspension culture, our scientists have developed a culture system that allows robust, stable growth and maintenance of pluripotency under defined, feeder-free, and xeno-free conditions. The Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium paired with a perfusion bioreactor system provides an ideal method for creating large amounts of high-quality, undifferentiated starting material. The formulation of this medium is based on the 2D medium, Cellartis DEF-CS 500 Xeno-Free Culture Medium, and cells growing in 2D can easily be transitioned to 3D for robust scale-up.